Purification and characterization of asparaginase II from Saccharomyces cerevisiae cloned in Pichia pastoris: a study on a possible antileukemic drug
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چکیده
Introduction Bacterial asparaginase obtained from Escherichia coli and Erwinia chrysanthemi are used as medicine to treat acute lymphocytic leukemia and non-Hodgkin lymphoma. Despite the therapeutic properties of such enzymes there have been reports on adverse reactions, eventually so severe as to impede some patients of using the medicine. Besides, the only drug Brazil used to import is no longer produced. Considering these two factors our proposition is to produce non-bacterial asparaginase.
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